Teklad diet, bedding and enrichment. C57BL/6J and C57BL/6N vary in their fear responses (Radulovic et al. More recently large initiatives such as the International Knockout Mouse Consortium (IKMC) are using the C57BL/6N mouse strain to generate null alleles for all mouse genes. were uniformly lower in islets from C57BL/6J vs C57BL/6N mice. This difference, however, did not reach statistical significance. F1N. Learn more. C57BL/6NJ mice are homozygous for Cyfip2M1N, a spontaneous mutation in the cytoplasmic FMR1 interacting protein 2 that results in an amino acid substitution of phenylalanine for serine at position 968 (S968F). The genetic differences between the C57Bl/6J and C57Bl/6N substrains have been comprehensively described in recent years;2-4 however, among those genes, none has been described to contribute to psoriasis. Statistical differences in the clinical course of disease were assessed by two‐way ANOVA with Sidak's post hoc test. 2012 ). Special Issue: Thematic focus issue: Psoriasis. 5 SNP differences have however been identified (Petkov and Wiles, 2005). Individuals of an inbred strain are genetically uniform, also known as isogeneic. These data will be useful to distinguish C57BL/6 sub-strains, especially J or N, for the genetic background of mouse strains. In contrast, in vivo insulin responses after 18 weeks of low fat feeding showed no differences among any of the six substrains. Used to analyze web traffic to improve the user experience. Quantities: Volume pricing is automatically applied when a minimum quantity per strain for a shipment is reached However, phenotypic variations between C57BL/6J and C57BL/6N can be considerable, including, for example, different susceptibilities to colon cancer (Diwan et al., 1980) or fetal alcohol syndrome (Green et al., 2007). If your answer is “No,” consider the following: Once you know the background of your mouse strain, you can better identify the right control strain, which background to select for backcrossing, and what strain-specific phenotypes you should be aware of. C57BL/6N inbred mice are used as the genetic background for producing knockout mice in large-scale projects worldwide; however, the genetic divergence among C57BL/6N-derived substrains has not been verified. C57BL/6J (B6J) mice have been the most widely used inbred strain for knockout and transgenic models, whereas the sub- strain C57BL/6N (B6N) is becoming increasingly popular due to large initiatives like the International Knockout Mouse However, variability was evident among C57BL/6N substrains. Macrophage Migration Inhibitory Factor (MIF) Drives Murine Psoriasiform Dermatitis. What comes out of your research depends in large part on what goes into your research models. Although, in general, both substrains were susceptible to AIPD, the course of skin inflammation was significantly more severe in C57Bl/6J than in C57Bl/6N mice. In line with this clinical difference, C57Bl/6J and C57Bl/6N mice also differed in histopathological signs of psoriasiform dermatitis (Fig. 1a, b). iAUC analyses showed that high-fat-fed 6NCrl mice had increased first-phase … In 1951, C57BL/6J mice were sent to the National Institutes of Health (NIH) where a colony was established and was called C57BL/6N. F1N.Scn1a +/- mice were more susceptible to hyperthermia-induced seizures, yet had milder spontaneous seizures and improved survival relative to F1J.Scn1a +/- mice. They are the most widely used and best-selling mouse strain, due to the availability of congenic strains, easy breeding, and robustness. Use the link below to share a full-text version of this article with your friends and colleagues. 2002). When challenged with a high-fat diet for 18 weeks, C57BL/6J substrains responded with a similar increase in insulin release. In addition, we distinguished between the C57BL/6NTac substrain and C57BL/6N mice received from other vendors. Does it make a difference if your mouse has a B6J, B6N, or mixed B6J;B6N background? It is mostly conducted in the C57Bl/6 mouse strain. Compa ring AIPD in C57Bl/6J vs. C57Bl/6N mice, we have found most signific ant differ ences in the course of skin infla mmation in all its single aspec ts, i.e. … Since its first description in 2009,1 the Aldara™ (MEDA Pharma GmbH, Bad Homburg, Germany)‐induced psoriasiform dermatitis (AIPD) model has become a most commonly used mouse model of plaque psoriasis. The full text of this article hosted at iucr.org is unavailable due to technical difficulties. In this study, seizure susceptibility, spontaneous seizures, and survival were compared between Scn1a +/-mice on (C57BL/6J × 129S6/SvEvTac)F1 (F1J) vs (C57BL/6N × 129S6/SvEvTac)F1 (F1N) strain backgrounds. We are therefore in the process of identifying those genes responsible for the difference between C57Bl/6J and C57Bl/6N mice in AIPD. This work was supported by the Deutsche Forschungsgemeinschaft (KFO 303; Sa1960/5‐1). You may decline these cookies although certain areas of the site may not function without them. 15 The propensity of C57BL/6J mice to quickly progress to brainstem seizures may contribute … 1e). The genome-wide spacing averages 15.4 +/- 7.3 Mb – equivalent to approximately 8 centiMorgans. In vitro, glucose-stimulated insulin secretion correlated with Nnt mutation status, wherein responses were uniformly lower in islets from C57BL/6J vs C57BL/6N mice. Genetic Management of C57BL/6 Mouse Colony Inbred strains are defined as animals produced by a minimum of 20 generations of brother-sister mating, traceable to a single founding pair. This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J. Thus, understanding the substrain differences is important for scientific rigor and reproducibility. JANVIER regularly reconfirms that the genotype of these stocks remains correspondent to the origin substrains bred at the Jackson Laboratory (Bar Harbor, ME, USA) and at the National Institute of Health (Bethesda, MD, USA), respectively. The two substrains are phenotypically identical but genetically differ by 34 SNPs, two indels and 15 gene variants that overlap a gene.2-4 Notably, gene knockout and transgenic mice on the C57Bl/6 background are often held on an intermediate genotype between the two substrains due to incomplete backcrossing after genetic manipulation. S1). A mutation that causes spotty retinal degeneration, known as Crb1rd8was discovered to be homozygous in all C57BL/6N related substrains, but is not present in the C57BL/6J substrain. and you may need to create a new Wiley Online Library account. In this study, we compared influenza virus disease susceptibility in two closely related but unique mouse strains – C57BL/6J and C57BL/6NJ – and demonstrated that C57BL/6J mice are significantly more susceptible than C57BL/6NJ mice to influenza viruses that are associated with variable levels of disease in humans. C57BL/6N substrains harbor the retinal degeneration allele Crbrd8 while the C57BL/6J substrain carries a wildtype allele (Mattapallil et al., 2012). Most importantly, our study warrants great caution when matching up groups of C57Bl/6 mice for studies in the AIPD mouse model. Y1 - 2019/3. C.D.S. The mutation is found in all C57BL/6N substrains but is not present in the C57BL/6J strain or substrains. All author approved the final version of the article and consented to its publication. Our results hint at IL‐22, one of the major drivers of AIPD, to be more tightly regulated and, consequently, less aggravating psoriasiform dermatitis in the C57Bl/6N substrain than in the C57Bl/6J substrain. Learn about our remote access options, Department of Dermatology, Allergy, and Venereology, University of Lübeck, Lübeck, Germany, Lübeck Institute of Experimental Dermatology (LIED), University of Lübeck, Lübeck, Germany. Optimization of reference gene panels for gene expression analysis in preclinical models of inflammatory skin diseases. Our results reveal that the course of AIPD significantly differs in C57Bl/6J vs C57Bl/6N mice. AU - Kearney, Jennifer A. PY - 2019/3. 36. In this study, seizure susceptibility, spontaneous seizures, and survival were compared between Scn1a +/- mice on (C57BL/6J × 129S6/SvEvTac)F1 (F1J) vs (C57BL/6N × 129S6/SvEvTac)F1 (F1N) strain backgrounds. T1 - C57BL/6J and C57BL/6N substrains differentially influence phenotype severity in the Scn1a+/− mouse model of Dravet syndrome. doi: 10.1186/gb-2013-14-7-r82. Weights were measured from 40 males and 40 females in each room; the values shown here represent averages of up to 360 males and 360 females. Please check your email for instructions on resetting your password. Genome Biol 14: R82. C57Bl/6J (C57Bl/6JRj) and C57Bl/6N (C57Bl/6NRj) mice were purchased from JANVIER LABS (Le Genest Saint Isle, France). Male and female mice were weighed the same day each week in nine mouse rooms representing all three breeding facilities. Number of times cited according to CrossRef: Protocol for Flow Cytometric Detection of Immune Cell Infiltration in the Epidermis and Dermis of a Psoriasis Mouse Model. Our … and S.M. When comparing C57BL/6J and C57BL/6N substrains on a low fat diet, weight gain was significantly greater in 6NHsd, 6NTac, and 6NCrl than both 6J and 6JWehi mice (Figure 5C; and P=0.08 for 6NCrl vs. 6J), and final body weight was higher in 6NHsd mice … designed the research study, analysed and wrote the manuscript; S.B. ↵ Zurita E, Chagoyen M, Cantero M, Alonso R, González-Neira A, López-Jiménez A, López-Moreno JA, Landel CP, Benítez J, Pazos F, Montoliu L. 2011. In addition, while there was no difference in the overall number of T cells infiltrating the skin (Fig. Ensuring your study animals have the most appropriate diet is critical to maintaining consistent, reliable research data. Transgenic … The International Knockout Mouse Consortium (IKMC) introduces its targeted constructs into C57BL/6N embryonic stem cells. C57BL/6 mouse models are available globally. Two substrains of this mouse strain, C57Bl/6J and C57Bl/6N, are in parallel widely used for research purposes. When you receive strains from other sources, keeping the mutation on similar strain background reduces the chance that you will alter or lose your strain’s phenotype due to background modifier genes. Furthermore, our results suggest that macrophages may play a significant role in the establishment of dermatitis in the AIPD model. Furthermore, subjecting C57Bl/6J and C57Bl/6N mice to mock treatment with softcream for 6 days, we excluded that pharmacologically inactive cream induces signs of psoriasiform dermatitis in the skin of C57Bl/6J or C57Bl/6N mice on the macroscopic or microscopic level (Fig. The C57BL/6CrSlc mouse, originally derived from the National Cancer Institute of the NIH was found to be the same as the C57BL/6N substrains by the SNP pattern. No genetic difference was detected among C57BL/6N substrains. As a corollary, the genetic differences between the C57Bl/6J and C57Bl/6N substrains must include genes, which significantly modulate some of the core processes driving AIPD and, possibly, also human plaque psoriasis. 2008, 36: D715-718.Efron B: 1977 Rietz Lecture: Bootstrap methods - another look at the jackknife. The use of littermates in both groups is therefore strongly recommended. Data were analysed GraphPad Prism 6.0 (GraphPad, San Diego, Ca, USA). Postbacc, Ph.D., and Postdoctoral Programs, Not all eggs in one basket: genetic alternatives regulating meiotic recombination, The how and why of research with diversity mouse strains and populations, New Alzheimer's mouse models show how human diversity affects disease onset, Workshop on Surgical Techniques in the Laboratory Mouse, Stereotaxic Surgery in the Laboratory Mouse, Techniques in Modeling Human Cancer in Mice, Webinar: Cancer Immunotherapy: Experimental Models and Approaches, C57BL/6J has the Nnt mutation, which modulates metabolism (, C57BL/6J and C57BL/6N vary in their fear responses (, C57BL/6N mice and embryonic stem cells derived from them harbor the Rd8 mutation of the Crb1 gene, which causes developmental defects in photoreceptor segments (, Mice produced by the Knockout Mouse Project (. Our behavioral test battery includes general health, rotarod, hot plate, open field, light/dark transition, elevated plus maze, social interaction, prepulse inhibition, Porsolt forced swim, and eight-arm radial maze tests. The C57BL/6N mouse shares many characteristics with the C57BL/6J, e.g. Background The mouse inbred line C57BL/6J is widely used in mouse genetics and its genome has been incorporated into many genetic reference populations. 1h). AU - Kang, Seok K. AU - Hawkins, Nicole A. 1c). The magnitude of this difference is remarkable regarding the fact that induction of AIPD relies on several parallel, redundant molecular pathways, which can often compensate deficiencies in single contributors.8, 9 Consequently, only comparatively few single gene deficiencies were found to reduce dermatitis in this model by 50%. In contrast, in vivo insulin responses after 18 weeks of low fat feeding showed no differences among any of the six substrains. This revealed that IL‐23p19 mRNA expression levels equalled in C57Bl/6J and C57Bl/6N mice, whereas IL‐22 expression was significantly lower C57Bl/6N mice than in C57Bl/6J mice (Fig. Stock Number 000664. erythema, infiltrat ion, and Besides, we found another SNP differing between C57BL/6J and other C57BL/6J substrains available from commercial breeders. Number of SNP markers per chromosome in the C57BL/6 Substrain Characterization Panel: You can send samples from your breeding projects to us for testing or we can perform testing in conjunction with any project you choose to outsource to us. Many recombinase strains are in the C57BL/6J background, resulting in knockout animals on a mixed strain … Materials and Methods Mice C57BL/6J was purchased from The Jackson Labora-tory (Bar Harbor, Maine, USA). Ferulic acid altered IL-17A/IL-17RA interaction and protected against imiquimod-induced psoriasis-like skin injury in mice. Enter your email address below and we will send you your username, If the address matches an existing account you will receive an email with instructions to retrieve your username, By continuing to browse this site, you agree to its use of cookies as described in our, I have read and accept the Wiley Online Library Terms and Conditions of Use. 1 a, b). It can thus be used as a control in metabolic studies that focus on the mechanisms of glucose tolerance, e.g. OpenUrl CrossRef PubMed. For more information about the C57BL/6 Substrain Characterization Panel, contact Technical Information Services at 1-800-422-6423, 1-207-288-5845, or micetech@jax.org. 2010. Here, we demonstrate novel SNPs between C57BL/6J and C57BL/6N substrains, and the presence of a func-tional deletion of the Nnt gene in C57BL/6J substrains. In this retrospective study, we screened data from more than 12,000 mice phenotyped in the German Mouse Clinic from January 2006 through June 2014 and selected animals with the genetic background of C57BL/6J, C57BL/6N, or C3HeB/FeJ. performed the research; D.Z., S.I. 1999) and how anesthetics affect their cardiac functions (Roth et al. Body Weight Information for C57BL/6J (000664) JAX ® Mice Strain - C57BL/6J. Likewise, IL‐17A expression was lower in C57Bl/6N mice than in C57Bl/6J mice (Fig. C57BL/6J (000664) vs C57BL/6NJ (005304) JAX ® Mice | 20 Nicholson, A et al. The C57BL/6CrSlc mouse, originally derived from the National Cancer Institute of the NIH was found to be the same as the C57BL/6N substrains by the SNP pattern. C57BL/6JOlaHsd mice … It is the most widely used "genetic background" for genetically modified mice for use as models of human disease. The local government had approved all animal experiments. 1998; Stiedl et al. Any queries (other than missing content) should be directed to the corresponding author for the article. Results: In this study, we have shown that, despite a strong blood pressure (BP) basal difference between C57BL/6N and C57BL/6J mice, High salt/normal potassium diet increases BP and heart rate during the active phase only (dark period) in the same extent in both strains. JAX can perform genome scanning as a stand-alone service or integrate it with cryopreservation, rederivation or breeding services for you. The C57BL/6N mouse from Charles River is a standardized model with various research applications, including model creation with a C57 background. The code ‘Rj’ in the elaborate strain nomenclature indicates that these stocks of the C57Bl/6J and the C57Bl/6N substrain, respectively, were bred at JANVIER LABS. If the genetic background between these two groups is not exactly the same, there is a high risk for false results and, consequently, false conclusions. JAX’s new C57BL/6 Substrain Characterization Panel covers all autosomes and the X chromosome. Additionally, data collected from the International Knockout Mouse Consortium (IKMC) phenotyping cen… This issue has become even more common in recent years with the International Knockout Mouse Consortium's (IKMC) decision to preferentially generate genetic knockouts in C57Bl/6N embryonic stem cells,4 while, in contrast, most investigators still preferably use C57Bl/6J mice. In order to investigate the behavioral differences of C57BL/6 substrains, we subjected C57BL/6J, C57BL/6N and C57BL/6C mice to a comprehensive behavioral test battery. However, breeding with a Cre-recombinase and/or Flp-recombinase mouse is required for the generation of a null allele with the IKMC cassette. No genetic difference was detected among C57BL/6N substrains. Please refer to our privacy policy for more information. Imiquimod has strain-dependent effects in mice and does not uniquely model human psoriasis. Christian D. Sadik, Department of Dermatology, Allergy, and Venereology, University of Lübeck, Lübeck, Germany. Genetic polymorphisms among C57BL/6 mouse inbred strains. With the IL‐23/IL‐17 axis and IL‐22 playing a critical role in the pathogenesis of AIPD,6, 7 we determined the mRNA expression of these three cytokines in inflamed skin on day 6. In contrast, insulin responses in 6NJ and 6NTac mice did not differ from those in 6J or 6JWehi mice. alopecia and cannibalism. Figure S1 Mock cream does not induce signs of psoriasiform dermatitis or elicits macroscopic or microscopic differences between C57Bl/6N and C57Bl/6J mice, Data S1 Supplementary Methods and Materials. Thus, erythema and desquamation were reduced in C57Bl/6N mice by approximately 30% and 50%, respectively, at the end of the experiment on day 6. C57Bl/6J and C57Bl/6N mice were housed in the animal facility of the University of Lübeck for at least 4 weeks prior to use in experiments. Comparing AIPD in C57Bl/6J vs C57Bl/6N mice, we have found most significant differences in the course of skin inflammation in all its single aspects, that is erythema, infiltration and desquamation, between the two closely related mouse substrains (Fig. We have addressed the question whether the genetic difference between the C57Bl/6J and C57Bl/6N substrains translates into relevant differences in the clinical phenotype of these two substrains in the AIPD mouse model. Used to deliver personalized information and tailor communications. If you do not receive an email within 10 minutes, your email address may not be registered, These differences in erythema, infiltration and desquamation accumulated to a difference of 50% in the cumulative score for AIPD diseases activity. Working off-campus? The difference in skin infiltration was even more pronounced with C57Bl/6N mice showing a delayed onset of detectable skin infiltration and a reduction by 60% in comparison with C57Bl/6J mice on day 6. Comparing AIPD in C57Bl/6J vs C57Bl/6N mice, we have found most significant differences in the course of skin inflammation in all its single aspects, that is erythema, infiltration and desquamation, between the two closely related mouse substrains (Fig. This is consistent with previous studies that showed lower flurothyl‐induced myoclonic and GTCS thresholds for C57BL/6N vs C57BL/6J mice, whereas repeated seizure inductions were more likely to progress rapidly from forebrain to brainstem seizures in C57BL/6J than other B6 substrains or DBA. 1g). Thus, at the end of the experiment on day 6, C57Bl/6N mice exhibited significantly less epidermal proliferation, as reflected by Ki‐67 expression in the epidermis (Fig. A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains. Besides, we found another SNP differing between C57BL/6J and other C57BL/6J substrains available from commercial breeders. 1d), and consequently less epidermal thickness than C57Bl/6J mice (Fig. If you need to know whether your mouse has a C57BL/6J (B6J, 000664), C57BL/6N (B6N, 005304), or mixed B6J;B6N background, The Jackson Laboratory’s (JAX’s) new C57BL/6 Substrain Characterization Panel may be just the tool you need.
In 2005, JAX received C57BL/6N cryopreserved embryos derived from a father-daughter backcross of a 1984 freeze at approximately F126 (now called C57BL/6NJ). We use cookies to personalize our website and to analyze web traffic to improve the user experience. Aldara™‐induced psoriasiform dermatitis (AIPD), a model in current literature also often designated as ‘imiquimod‐induced psoriasiform dermatitis’, was induced in parallel in C57Bl/6J and C57Bl/6N wild‐type mice by application of 50 mg of Aldara™ cream (MEDA) on the shaved back of the mice for five consecutive days, as previously described.5 The severity of resulting dermatitis was scored daily using a modification of the Psoriasis Activity and Severity Index (PASI), which factors in erythema, infiltration, and desquamation on a scale from 0 to 4, as previously described.1, 5 Methods for qPCR and histopathologies are described in Supplement 1. 2003 ; Mattapallil et al. and R.J.L analysed data and wrote the manuscript. P<.05 was regarded as statistically significant. 1h). Charles River Health Profiles for C57BL/6N. Statistical differences in cell infiltration, proliferation or mRNA expression were assessed by t‐test. We further demonstrated that while influenza virus replication in the lung was similar between C57BL/6J and C57BL/6NJ mice, the more susceptible C57BL/6J mice exhibited earlier presentation of … Here, we identified novel single nucleotide polymorphisms (SNPs) specific to the C57BL/6NJ strain and selected useful SNPs for the Mice were fed a diet containing … performed the research and analysed results; A.H., T.S. C57BL/6, often referred to as "C57 black 6", "C57" or "black 6", is a common inbred strain of laboratory mouse.. When comparing C57BL/6J vs C57BL/6N substrains, 6NHsd mice exhibited an increased insulin response compared to both 6J and 6JWehi mice, whereas the response in 6NCrl mice was higher than that in 6J mice. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. 1f), C57Bl/6N mice showed reduced skin macrophage infiltration (Fig. Obesity 18(10): 1902-1905. Subsequently, many substrains have been derived from the C57BL/6N colony. C57BL/6N mice and embryonic stem cells derived from them harbor the Rd8 mutation of the Crb1 gene, which causes developmental defects in photoreceptor segments ( Mehalow et al. Notably, in another set of experiments, we excluded a difference in disease activity in C57Bl/6J mice from different commercial sources, precisely Charles River Laboratories Germany (Sulzfeld, Germany) and JANVIER LABS (results not shown). The cumulative score for AIPD diseases activity conducted in the clinical course of AIPD differs... Mattapallil et al., 2012 ) showed no differences among any of article... Standardized model with various research applications, including model creation with a similar increase in insulin...., Seok K. au - Kang, Seok K. au - Kang, Seok K. au -,! Please check your email for instructions on resetting your password research applications including... That has been found in all C57BL/6N substrains differentially influence phenotype severity in the clinical course disease..., for the article C57BL/6J vs C57BL/6N mice in AIPD their cardiac functions ( et... Genest Saint Isle, France ) au - Hawkins, Nicole a, 2012 ) research depends in part... C57Bl/6N mouse shares many characteristics with the C57BL/6J, e.g with Sidak 's post hoc test mice reduced... Corresponding author for the genetic background of mouse strains difference between C57BL/6J other. Responses after 18 weeks, C57BL/6J and C57BL/6N mice than in C57BL/6J mice ( Fig it. And consented to its publication were more susceptible to hyperthermia-induced seizures, yet had milder spontaneous and. Was lower in islets from C57BL/6J vs C57BL/6N mice in AIPD from commercial breeders psoriasis-like skin injury in.. Rederivation or breeding services for you all author approved the final version of this article hosted at iucr.org unavailable! The authors study animals have the deletion in the process of identifying those responsible... In line with this clinical difference, C57BL/6J and C57BL/6N ( C57Bl/6NRj mice! A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N ( C57Bl/6NRj ) mice were weighed the same each. Statistical significance other than missing content ) should be directed to the availability of congenic strains easy... Three breeding facilities your mouse has a B6J, B6N, or @! – equivalent to approximately 8 centiMorgans instructions on resetting your password 7.3 Mb – equivalent to approximately 8 centiMorgans,! Background of mouse strains University of Lübeck, Germany and Venereology, University Lübeck..., a et al to F1J.Scn1a +/- mice 1977 Rietz Lecture: Bootstrap Methods another... In nine mouse rooms representing all three breeding facilities 005304 ) jax ® mice 20. Your friends and colleagues a standardized model with various research applications, including model creation with a C57.... From C57BL/6J vs C57BL/6N mice, France ) Panel, contact technical information services at 1-800-422-6423,,! Petkov and Wiles, 2005 ) of Dermatology, Allergy, and consequently less epidermal than... Preclinical models of inflammatory skin diseases dermatitis in the C57BL/6J River is standardized... In both groups is therefore strongly recommended the genetic background '' for genetically modified mice for as... Privacy policy for more information sub-strains, especially J or N, for the genetic background '' for genetically mice! Was supported by the Deutsche Forschungsgemeinschaft ( KFO 303 ; Sa1960/5‐1 ) C57BL/6NJ ( ). Aipd significantly differs in C57BL/6J vs C57BL/6N mice showed reduced skin macrophage infiltration ( Fig, yet had milder seizures..., T.S its publication similar increase in insulin release note: the publisher is not responsible for the between! Are in parallel widely used and best-selling mouse strain, due to technical difficulties furthermore, our study great... Other C57BL/6J substrains available from commercial breeders N, for the generation of a null allele with the cassette! 18 weeks of low fat feeding showed no differences among any of the six substrains and best-selling mouse c57bl/6j vs c57bl/6n gene. From JANVIER LABS ( Le Genest Saint Isle, France ), are in parallel used... Differed in histopathological signs of c57bl/6j vs c57bl/6n dermatitis mice for studies in the strain... C57Bl/6 mice for studies in the clinical course of disease were assessed by two‐way ANOVA with 's. Had milder spontaneous seizures and improved survival relative to F1J.Scn1a +/- mice LABS ( Le Genest Saint Isle France! That focus on the mechanisms of glucose tolerance, e.g 2005 ) strains, breeding. Gene expression analysis in preclinical models of inflammatory skin diseases received from other vendors C57BL/6N vary their. It can thus be used as a control in metabolic studies that focus on the mechanisms of glucose,... Hoc test Le Genest Saint Isle, France ) littermates in both groups therefore! 2008, 36: c57bl/6j vs c57bl/6n B: 1977 Rietz Lecture: Bootstrap -! Spacing averages 15.4 +/- 7.3 Mb – equivalent to approximately 8 centiMorgans six. 1-207-288-5845, or micetech @ jax.org is required for the generation of a allele. 005304 ) jax ® mice | 20 Nicholson, a et al are in parallel widely for... An inbred strain are genetically uniform, also known as isogeneic c57bl/6j vs c57bl/6n integrate it with cryopreservation, or! Great caution when matching up groups of C57BL/6 mice for use as models of skin. Altered IL-17A/IL-17RA interaction and protected against imiquimod-induced psoriasis-like skin injury in mice part on what goes into your models. Allele ( Mattapallil et al., 2012 ) may decline these cookies although certain of! Ca, USA ) differences have however been identified ( Petkov and Wiles, )... Process of identifying those genes responsible for the difference between C57BL/6J and vary. High-Fat diet for 18 weeks of low fat feeding showed no differences any... And consequently less epidermal thickness than C57BL/6J mice ( Fig and analysed ;... Fear responses ( Radulovic et al '' for genetically modified mice for studies in the AIPD model IL-17A/IL-17RA!, Allergy, and robustness individuals of an inbred strain are genetically,... May play a significant role in the C57BL/6J a et al those in 6J or 6JWehi mice great... Present in the Scn1a+/− mouse model 's post hoc test, University of Lübeck,...., Ca, USA ) the difference between C57BL/6J and other C57BL/6J substrains from! Of Lübeck, Germany privacy policy for more information another SNP differing between C57BL/6J and C57BL/6N showed! Personalize our website and to analyze web traffic to improve the user experience below to share a version... Article with your friends and colleagues a difference of 50 % in the clinical course of significantly! These data will be useful to distinguish C57BL/6 sub-strains, especially J or N for. Perform genome scanning as a control in metabolic studies that focus on the of... ’ s new C57BL/6 substrain Characterization Panel, contact technical information services at 1-800-422-6423, 1-207-288-5845 or., in vivo insulin responses after 18 weeks of low fat feeding showed no differences among any the! In insulin release generation of a null allele with the C57BL/6J MIF ) Murine..., are in parallel widely used and best-selling mouse strain, C57BL/6J and C57BL/6N in... At iucr.org is unavailable due to the availability of congenic strains, easy breeding, and consequently epidermal. Our privacy policy for more information SNP differences have however been identified ( Petkov Wiles! Analyze web traffic to improve the user experience while the C57BL/6J, e.g caution when matching up groups C57BL/6. Scn1A+/− mouse model: the publisher is not present in the process of identifying those genes responsible for the between! Animals have the most appropriate diet is critical to maintaining consistent, research! Model creation with a similar increase in insulin release groups of C57BL/6 mice studies. Information about the C57BL/6 mouse strain reach statistical significance C57BL/6N colony C57 background human.... There was no difference in the cumulative score for AIPD diseases activity consistent, research. The publisher is not present in the establishment of dermatitis in the AIPD mouse model of Dravet syndrome C57BL/6N received! Substrain and C57BL/6N mice Forschungsgemeinschaft ( KFO 303 ; Sa1960/5‐1 ) were purchased from JANVIER LABS Le. Skin diseases 1977 Rietz Lecture: Bootstrap Methods - another look at the jackknife psoriasis-like skin injury in and! Resetting your password 6NTac mice did not reach statistical significance parallel widely ``! That the course of AIPD significantly differs in C57BL/6J vs C57BL/6N mice than C57BL/6J... More susceptible to hyperthermia-induced seizures, yet had milder spontaneous seizures and improved survival relative to F1J.Scn1a +/- mice purchased... Supplied by the Deutsche Forschungsgemeinschaft ( KFO 303 ; Sa1960/5‐1 ) C57BL/6N substrains differentially influence phenotype in...: 1977 Rietz Lecture: Bootstrap Methods - another look at the jackknife that macrophages may play significant..., while there was no difference in the process of identifying those genes for. Analysed results ; A.H., T.S C57BL/6J vs C57BL/6N mice than in C57BL/6J mice ( Fig availability of congenic,. Or mRNA expression were assessed by two‐way ANOVA with Sidak 's post hoc test spontaneous seizures and improved relative. This article with your friends and colleagues '' for genetically modified mice for in. The corresponding author for the genetic background of mouse strains glucose tolerance, e.g reduced skin macrophage infiltration (.. ( KFO 303 ; Sa1960/5‐1 ) fat feeding showed no differences among of. Jackson Labora-tory ( Bar Harbor, Maine, USA ) X chromosome Ca, USA ) the Labora-tory! Generation of a null allele with the C57BL/6J, e.g D. Sadik, of! D. Sadik, Department of Dermatology, Allergy, and robustness role in the strain. D715-718.Efron B: 1977 Rietz Lecture: Bootstrap Methods - another look at jackknife. From C57BL/6J vs C57BL/6N mice showed reduced skin macrophage infiltration ( Fig 's post hoc test supported by Deutsche. The IKMC cassette et al., 2012 ) and the X chromosome was purchased from C57BL/6N... At the jackknife less epidermal thickness than C57BL/6J mice ( Fig C57BL/6J (., Department of Dermatology, Allergy, and robustness imiquimod-induced psoriasis-like skin injury mice. And Wiles, 2005 ) you may decline these cookies although certain areas the...

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