A blood smear evaluates red blood cells (RBC morphology) white blood cells (WBC) and platelets to help diagnose The manual peripheral blood smear Whilst the manual differential count is generally considered to be superior to an analyser generated count for pathologi-cal samples, this only holds true under very strict conditions. Improper timing of staining or buffering, Deteriorated reagent. You can change your ad preferences anytime. Two push-type peripheral blood smears suitable for characterization of cellular blood elements. Normal, mature RBCs are biconcave, disc-shaped, anuclear cells measuring approximately 7-8 microns in diameter on a peripheral blood smear with an internal volume of 80-100 femtoliters (fL). Clinical Case Study Fatigued: A Case on Blood Download Case Study with Worksheet Harold is a 67-year-old male who has had regular physical exams, is a non-smoker, and who has been in good health for most of his life. A good wedge smear should cover half to three fourths of the slide, should show gradual transition from a thick to a thin end with a feathered edge. It consists of an outer body tube and an inner draw tube which can slide inside the outer, eye piece and lower part carrying a revolving … Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood … State the appropriate sample used for preparing a peripheral blood smear. Osmotic fragility & rbc membrane defects 050916, No public clipboards found for this slide. Good Blood Smear - Margin free with distinct lateral edge, Smooth Appearance, Placed centrally on the slide, Has uniform distribution of leukocytes, Whole drop of blood is picked up and spread, Has gradual transition from thick to thin ,When held up to light has a rainbow appearance. Desirable qualities of a blood smear include: i. Peripheral 11. Bacteria, Babesia or Malaria, Stain Precipitates. You can have a pap smear with an IUD in place. Prolonged storage of anticoagulated blood specimen, Delay in spreading the blood drop, Dirty and poor quality glass slide, Inappropriate size of blood drop, Improper angle of the pusher slide, Improper pressure/speed, High Humidity. The peripheral blood film remains a very useful tool in hematological assessment. Describe the appearance of a well-prepared peripheral blood film, recognize a description of a slide that is consistent or inconsistent with that appearance, and 4. Pathophysiology There are various sizes and shapes of RBC seen in the peripheral blood smear like: Normocytic when the size is normal (7 to 8 µm). There are many reasons why your doctor may order a blood smear. It should have a smooth appearance and have no ridges, holes, lines, streaks or clumps. Large granular lymphocytes noted, a representation of natural killer cells or cytotoxic T lymphocytes. In peripheral blood film (PBF), neutrophils showed characteristic C-shaped, fetus-like nuclei, elongated nucleoplasm, and ring-shaped nuclei. It is often done as part of or along with a complete blood count (CBC). Does not Peripheral blood smear of a 38-year-old female with long-standing Crohn’s disease (CD) and development of microcytic anemia. A lymphocyte is a type of white blood cell in the vertebrate immune system. 2. An IUD does not interfere with the ability to get a good sample. Parts of the Compound Microscope Tube It is cylindrical tube through which the light from an illuminated object traverses enroute its formation of an image. 2.Smear should be tongue shaped . Bone Marrow (not performed by IDC). Characteristics of good smear : 1.Surface should be even and uniform ,margins should not extend to the side of the slide. The smear shows numerous target cells and a spur cell (top right). List the characteristics of a good blood smear. 3.Film should not be too thin or too thick . A polychrome stain, contains methylene blue and eosine dye dissolved in methyl alcohol. 4. List the characteristics of a good blood smear Good Blood Smear - Margin free with distinct lateral edge, Smooth Appearance, Placed centrally on the slide, Has uniform distribution of leukocytes, Whole drop of blood is picked up and spread, Has gradual transition from thick to thin ,When held up to light has a rainbow appearance. Exercise 9: Preparation of Peripheral Blood Smears Blood viscosity (thickness of blood) will vary from patient to patient. With the advent of hematological automated machines, complete and highly accurate hematological data and parameters like complete blood count or CBC can be obtained within a few minutes. If red blood cells appear blueish or green indicates stain or buffer is too alkaline, smear is too thick, Prolonged Staining. All liver function tests were abnormal indicating that the target cells are due to liver disease secondary to CD. Venipuncture with anticoagulant (Use EDTA collected specimen within 1 hour). Describe the optimal area for evaluating and enumerating blood cells on a well stained smear. blood smear. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Different Dyes stain various cell componets with different color. A blood smear is used when a CBC with differential shows the presence of abnormal or immature cells. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… A blood smear is often used as a follow-up test to abnormal results on a total blood count (CBC) to evaluate the various types of blood cells. Now customize the name of a clipboard to store your clips. 1. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Making a great blood smear (or blood film as some call it) is an important skill for any veterinary professional, even if your hospital does not require differentials to be done on a regular basis.It’s a great way to impress your colleagues! Amp ; RBC membrane defects 050916, no public clipboards found for this.. Membrane defects 050916, no public clipboards found for this slide development of microcytic anemia you browsing... When a CBC with differential shows the presence of abnormal or immature cells rbcs are not quite touching and no. Or clumps and eosine dye dissolved in methyl alcohol timing of staining or buffering, Deteriorated reagent stained... Is used when a CBC with differential shows the presence of abnormal or immature cells a to. 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